Hotspot

petA-psbJ

LSC · 60022-60500 · 479 bp

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Pi 0.0483

MarkerSeek score 80.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 42
pi: 0.0483
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTCTTTCCCCGGACCCTTA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1530-1549 bp	1.0	84.3
petA-psbJ_p2	TATTCTTTCCCCGGACCCTT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1531-1550 bp	1.0	84.3
petA-psbJ_p3	TTCTTTCCCCGGACCCTTAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1529-1548 bp	1.0	84.3
petA-psbJ_p4	ATTCTTTCCCCGGACCCTTA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1535-1550 bp	0.75	71.3
petA-psbJ_p5	TTCTTTCCCCGGACCCTTAT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1534-1549 bp	0.75	71.3