Hotspot

trnG-UCC-trnR-UCU

LSC · 9464-9782 · 319 bp

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Pi 0.0956
MarkerSeek score 80.50
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
319
variable_sites
50
pi
0.0956
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 TTCCAAGCTAATGATGCGGG AGAAGACCTATGTCCTATCCA 50.0 42.9 58.3 54.6 377-420 bp 1.0 54.2
trnG-UCC-trnR-UCU_p2 GCTAATGATGCGGGTTCGAT AGAAGACCTATGTCCTATCCA 50.0 42.9 58.5 54.6 371-414 bp 1.0 52.9
trnG-UCC-trnR-UCU_p3 CCAAGCTAATGATGCGGGTT AGAAGACCTATGTCCTATCCA 50.0 42.9 58.6 54.6 375-418 bp 1.0 52.0
trnG-UCC-trnR-UCU_p4 AATGATGCGGGTTCGATTCC AGAAGACCTATGTCCTATCCA 50.0 42.9 58.7 54.6 368-411 bp 1.0 52.0
trnG-UCC-trnR-UCU_p5 AAGCTAATGATGCGGGTTCG AGAAGACCTATGTCCTATCCA 50.0 42.9 58.7 54.6 373-416 bp 1.0 52.0