Hotspot

trnS-GCU-trnG-UCC

LSC · 8362-8713 · 352 bp

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Pi 0.0638
MarkerSeek score 80.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
352
variable_sites
41
pi
0.0638
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCACCGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 57.9 55.5 404-679 bp 1.0 61.7
trnS-GCU-trnG-UCC_p2 AATCCACCGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 57.9 55.5 403-678 bp 1.0 61.7
trnS-GCU-trnG-UCC_p3 GCAGAAAGGGAAAGAGAGGG ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 464-739 bp 1.0 60.8
trnS-GCU-trnG-UCC_p4 CTCGTACAGCGGATTAGCAA ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 421-696 bp 1.0 60.7
trnS-GCU-trnG-UCC_p5 AACTCGTACAGCGGATTAGC ACGAATCACACTTTTACCACT 50.0 38.1 57.8 55.5 423-698 bp 1.0 60.6