Hotspot

rps16-trnQ-UUG

LSC · 5948-7080 · 1133 bp

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Pi 0.0557
MarkerSeek score 84.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1133
variable_sites
115
pi
0.0557
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AATCCTGTCCTTCAAGTCGC GTTCGAATCCTTCCGTTCCA 50.0 50.0 57.9 57.9 1186-1401 bp 1.0 89.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTTCCA 45.0 50.0 58.1 57.9 1173-1388 bp 1.0 89.3
rps16-trnQ-UUG_p3 AATCCTGTCCTTCAAGTCGC TTCGAATCCTTCCGTTCCAG 50.0 50.0 57.9 57.6 1185-1400 bp 1.0 87.3
rps16-trnQ-UUG_p4 AATCCTGTCCTTCAAGTCGC AGGTTCGAATCCTTCCGTTC 50.0 50.0 57.9 57.6 1188-1403 bp 1.0 87.2
rps16-trnQ-UUG_p5 AATCCTGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTT 50.0 50.0 57.9 57.6 1189-1404 bp 1.0 87.2