Hotspot

rps19

LSC · 89938-90216 · 279 bp

Back to Thepparatia

Pi 0.00358

MarkerSeek score 41.10

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 1
pi: 0.00358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AGCGCATATCTGTCAAGTGG	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.1	58.1	929-937 bp	1.0	79.9
rps19_p2	AGCGCATATCTGTCAAGTGG	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.1	58.1	1127-1135 bp	1.0	79.7
rps19_p3	AGCGCATATCTGTCAAGTGG	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.1	58.1	1209-1217 bp	1.0	79.6
rps19_p4	TGCTGCTGCAGAATAAACCA	AAAAGTTTGGGTAGAGCCGG	45.0	50.0	58.1	58.1	936-944 bp	1.0	79.6
rps19_p5	AGCGCATATCTGTCAAGTGG	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.1	58.1	1200-1208 bp	1.0	79.5