Hotspot

trnL-UAG-rpl32

SSC · 131078-132190 · 1113 bp

Back to Thepparatia

Pi 0.00181

MarkerSeek score 60.20

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1113
variable_sites: 2
pi: 0.00181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	CGGCGTTGAAAGCTTTTTCA	50.0	45.0	58.0	58.2	1224-1228 bp	1.0	79.1
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	CGGCGTTGAAAGCTTTTTCA	50.0	45.0	58.0	58.2	1225-1229 bp	1.0	79.1
trnL-UAG-rpl32_p3	TCCTAAGAGCAGCGTGTCTA	CGGCGTTGAAAGCTTTTTCA	50.0	45.0	57.9	58.2	1234-1238 bp	1.0	78.1
trnL-UAG-rpl32_p4	AGCGTGTCTACCGATTTCAC	AAGAAAGGATATTGGGCGGC	50.0	50.0	58.0	58.3	1240-1244 bp	1.0	77.9
trnL-UAG-rpl32_p5	CAGCGTGTCTACCGATTTCA	AAGAAAGGATATTGGGCGGC	50.0	50.0	58.0	58.3	1241-1245 bp	1.0	77.9