Hotspot

rps19-rpl2

IRb · 90217-90360 · 144 bp

Back to Thepparatia

Pi 0.0294

MarkerSeek score 71.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 144
variable_sites: 4
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	281-289 bp	1.0	82.2
rps19-rpl2_p2	ATGGTAGATGCTCTGGACCA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	57.8	58.1	282-290 bp	1.0	81.1
rps19-rpl2_p3	TGGTAGATGCTCTGGACCAA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	339-347 bp	1.0	80.7
rps19-rpl2_p4	TGGTAGATGCTCTGGACCAA	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.0	58.3	283-291 bp	1.0	80.7
rps19-rpl2_p5	ATGGTAGATGCTCTGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.8	57.8	340-348 bp	1.0	79.6