Hotspot

rpl16-rps3

LSC · 87331-88694 · 1364 bp

Back to Thepparatia

Pi 0.000744
MarkerSeek score 60.60
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1364
variable_sites
1
pi
0.000744
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 ACCGAAACAAATGCGATTACC GAGGGTAGGGTTCCTCTACA 42.9 55.0 57.5 57.2 1560-1580 bp 1.0 62.5
rpl16-rps3_p2 ACCGAAACAAATGCGATTACC CTCTACAAACCATTGGAGCGA 42.9 47.6 57.5 58.0 1547-1567 bp 1.0 61.0
rpl16-rps3_p3 ACCGAAACAAATGCGATTACC GAGGGTAGGGTTCCTCTACAA 42.9 52.4 57.5 57.9 1560-1580 bp 1.0 60.3
rpl16-rps3_p4 CCGAAACAAATGCGATTACCT GAGGGTAGGGTTCCTCTACA 42.9 55.0 57.2 57.2 1559-1579 bp 1.0 60.3
rpl16-rps3_p5 ACCGAAACAAATGCGATTACC TCTACAAACCATTGGAGCGA 42.9 45.0 57.5 56.9 1546-1566 bp 1.0 60.1