Hotspot

psbH-petB

LSC · 80376-81320 · 945 bp

Back to Thepparatia

Pi 0.00423

MarkerSeek score 61.20

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 4
pi: 0.00423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	CGCAATGGCTTTATTTGCGA	TCTGCAATCGCCTGAATCTC	45.0	50.0	58.1	58.1	1083 bp	1.0	79.1
psbH-petB_p2	TCGCAATGGCTTTATTTGCG	TCTGCAATCGCCTGAATCTC	45.0	50.0	58.1	58.1	1084 bp	1.0	79.1
psbH-petB_p3	AACGACTCCTTTGATGGGTG	TCTGCAATCGCCTGAATCTC	50.0	50.0	57.8	58.1	1104 bp	1.0	78.4
psbH-petB_p4	GGAACGACTCCTTTGATGGG	TCTGCAATCGCCTGAATCTC	55.0	50.0	58.3	58.1	1106 bp	1.0	77.8
psbH-petB_p5	GGGAACGACTCCTTTGATGG	TCTGCAATCGCCTGAATCTC	55.0	50.0	58.3	58.1	1107 bp	1.0	77.8