Hotspot

psbE-petL

LSC · 70058-70935 · 878 bp

Back to Thepparatia

Pi 0.00342

MarkerSeek score 60.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 3
pi: 0.00342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AATAACCAACCCGCGATGAA	TGAGGTTATAGTTAAAGCCGCT	45.0	40.9	57.9	57.3	1045-1046 bp	1.0	57.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	962-963 bp	1.0	57.0
psbE-petL_p3	TGCTGACGAATAACCAACCC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.2	57.3	1053-1054 bp	1.0	56.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TGAGGTTATAGTTAAAGCCGCT	55.0	40.9	58.2	57.3	957-958 bp	1.0	56.7
psbE-petL_p5	AACCAACCCGCGATGAATAG	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.3	57.3	1042-1043 bp	1.0	55.6