Hotspot

psbZ-trnG-GCC

LSC · 38590-39312 · 723 bp

Back to Thepparatia

Pi 0.0112

MarkerSeek score 66.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 8
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	872-880 bp	1.0	78.6
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	870-878 bp	1.0	78.5
psbZ-trnG-GCC_p3	CTCCTGATGGTTGGTTGAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	866-874 bp	1.0	73.3
psbZ-trnG-GCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	871-879 bp	1.0	72.7
psbZ-trnG-GCC_p5	TCTCCTGATGGTTGGTTGAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	867-875 bp	1.0	70.9