Hotspot

ccsA-ndhD

SSC · 120452-120770 · 319 bp

Back to Theobroma

Pi 0.00629

MarkerSeek score 36.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 319
variable_sites: 3
pi: 0.00629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTATTTTGGGGTCA	GTCCTCTCACTATCGGGTGA	40.9	55.0	57.2	57.9	456-457 bp	1.0	57.8
ccsA-ndhD_p2	TGGATATGCTATTTTGGGGTCA	TATCGGGTGAGAAGGTCGAA	40.9	50.0	57.2	57.9	446-447 bp	1.0	57.2
ccsA-ndhD_p3	TGGATATGCTATTTTGGGGTCA	ATCCGGATTTCGTCCTCTCA	40.9	50.0	57.2	58.2	467-468 bp	1.0	56.5
ccsA-ndhD_p4	TGGATATGCTATTTTGGGGTCA	CTATCGGGTGAGAAGGTCGA	40.9	55.0	57.2	58.3	447-448 bp	1.0	55.7
ccsA-ndhD_p5	TGGATATGCTATTTTGGGGTCA	TTCGTCCTCTCACTATCGGG	40.9	55.0	57.2	58.3	459-460 bp	1.0	55.7