Hotspot

trnP-TGG-psaJ

LSC · 71158-71525 · 368 bp

Back to Theobroma

Pi 0.00725
MarkerSeek score 52.80
Primer available yes
Label trnP-TGG-psaJ

Feature Metadata

label_name
trnP-TGG-psaJ
feature_type
igs
region
LSC
length_bp
368
variable_sites
4
pi
0.00725
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-TGG-psaJ_p1 ACCCGTGACATTTTGTACCC AAACCCGCTAAAGAACCGAA 50.0 45.0 58.1 57.7 497 bp 1.0 77.8
trnP-TGG-psaJ_p2 CCCGTGACATTTTGTACCCA AAACCCGCTAAAGAACCGAA 50.0 45.0 58.1 57.7 496 bp 1.0 77.8
trnP-TGG-psaJ_p3 ACCCGTGACATTTTGTACCC CCGCTAAAGAACCGAACCAT 50.0 50.0 58.1 58.3 493 bp 1.0 77.7
trnP-TGG-psaJ_p4 CCCGTGACATTTTGTACCCA CCGCTAAAGAACCGAACCAT 50.0 50.0 58.1 58.3 492 bp 1.0 77.7
trnP-TGG-psaJ_p5 ACAGGATTTGAACCCGTGAC AAACCCGCTAAAGAACCGAA 50.0 45.0 58.1 57.7 508 bp 1.0 77.7