Hotspot

ndhC-trnV-UAC

LSC · 53989-55211 · 1223 bp

Back to Theobroma

Pi 0.0072
MarkerSeek score 65.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1223
variable_sites
13
pi
0.0072
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACCCTTTCTAATCGGGGCTA CGGTTCAAGTCCGTATAGCC 50.0 55.0 57.8 58.2 1357-1373 bp 1.0 79.8
ndhC-trnV-UAC_p2 CGGGGCTAAAACTCCAGAAA CGGTTCAAGTCCGTATAGCC 50.0 55.0 57.8 58.2 1345-1361 bp 1.0 79.7
ndhC-trnV-UAC_p3 ACCCTTTCTAATCGGGGCTA GGTTCAAGTCCGTATAGCCC 50.0 55.0 57.8 57.8 1356-1372 bp 1.0 79.1
ndhC-trnV-UAC_p4 CGGGGCTAAAACTCCAGAAA GGTTCAAGTCCGTATAGCCC 50.0 55.0 57.8 57.8 1344-1360 bp 1.0 79.0
ndhC-trnV-UAC_p5 ATCGGGGCTAAAACTCCAGA CGGTTCAAGTCCGTATAGCC 50.0 55.0 58.4 58.2 1347-1363 bp 1.0 77.9