Hotspot

trnS-GCT-trnG-GCC

LSC · 8240-9095 · 856 bp

Back to Theobroma

Pi 0.00702
MarkerSeek score 45.70
Primer available yes
Label trnS-GCT-trnG-GCC

Feature Metadata

label_name
trnS-GCT-trnG-GCC
feature_type
igs
region
LSC
length_bp
856
variable_sites
9
pi
0.00702
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCT-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TGTTCGGAATATGAATCAAACCG 50.0 39.1 58.0 57.5 980-988 bp 1.0 53.0
trnS-GCT-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TGTTCGGAATATGAATCAAACCG 50.0 39.1 58.0 57.5 979-987 bp 1.0 52.9
trnS-GCT-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 924-932 bp 1.0 52.5
trnS-GCT-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 923-931 bp 1.0 52.5
trnS-GCT-trnG-GCC_p5 CGCACAACGGATTAGCAATC TGTTCGGAATATGAATCAAACCG 50.0 39.1 57.9 57.5 995-1003 bp 1.0 52.2