Hotspot

petB

LSC · 71544-72949 · 1406 bp

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Pi 0.00322

MarkerSeek score 52.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1406
variable_sites: 20
pi: 0.00322
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1493-1521 bp	1.0	69.2
petB_p2	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1493-1521 bp	1.0	68.8
petB_p3	GGTAGTTCGACCGTGGATTT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1499-1527 bp	1.0	68.4
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1498-1526 bp	1.0	67.0
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1499-1527 bp	1.0	67.0