Hotspot

ccsA-ndhD

SSC · 108476-108655 · 180 bp

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Pi 0.0197

MarkerSeek score 55.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 180
variable_sites: 11
pi: 0.0197
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCGCACTTGTAGCTTCGATA	TCCGTTGATAGGGTAGAGGT	50.0	50.0	57.8	56.9	322-342 bp	1.0	76.3
ccsA-ndhD_p2	CCGCACTTGTAGCTTCGATA	TCCGTTGATAGGGTAGAGGTT	50.0	47.6	57.8	57.6	322-342 bp	1.0	74.3
ccsA-ndhD_p3	ATTCCGCACTTGTAGCTTCG	TCCGTTGATAGGGTAGAGGT	50.0	50.0	58.6	56.9	325-345 bp	1.0	72.8
ccsA-ndhD_p4	CGAATTCCGCACTTGTAGCT	TCCGTTGATAGGGTAGAGGT	50.0	50.0	58.6	56.9	328-348 bp	1.0	72.8
ccsA-ndhD_p5	CCGCACTTGTAGCTTCGATA	ATCCGTTGATAGGGTAGAGGT	50.0	47.6	57.8	57.4	323-343 bp	1.0	72.5