Hotspot

rpl32-trnL-UAG

SSC · 106822-107352 · 531 bp

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Pi 0.0113

MarkerSeek score 53.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 531
variable_sites: 22
pi: 0.0113
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTTTCTGGGCAACAAGC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.4	58.0	619-638 bp	1.0	82.0
rpl32-trnL-UAG_p2	GGTTTTTCTGGGCAACAAGC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.4	58.1	622-641 bp	1.0	81.5
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	592-611 bp	1.0	81.3
rpl32-trnL-UAG_p4	GGTTTTTCTGGGCAACAAGC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	585-604 bp	1.0	80.9
rpl32-trnL-UAG_p5	GGTTTTTCTGGGCAACAAGC	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.4	58.2	606-625 bp	1.0	80.6