Hotspot

ndhC-trnV-UAC

LSC · 50624-51425 · 802 bp

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Pi 0.0078

MarkerSeek score 63.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 19
pi: 0.0078
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	910-957 bp	1.0	83.0
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.0	57.9	1003-1050 bp	1.0	82.6
ndhC-trnV-UAC_p3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	911-958 bp	1.0	81.1
ndhC-trnV-UAC_p4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	909-956 bp	1.0	80.5
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	50.0	45.0	58.3	57.9	1002-1049 bp	1.0	80.1