Hotspot

petN-rpoB

LSC · 18085-19816 · 1732 bp

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Pi 0.00723

MarkerSeek score 57.70

Primer available yes

Label petN-rpoB

Feature Metadata

label_name: petN-rpoB
feature_type: igs
region: LSC
length_bp: 1732
variable_sites: 42
pi: 0.00723
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-rpoB_p1	AGCCCAAGCGAGACTTACTA	CCGGGTATTGTGGACATTCC	50.0	55.0	58.2	58.3	1576-1812 bp	1.0	82.1
petN-rpoB_p2	AGCCCAAGCGAGACTTACTA	CGGGTATTGTGGACATTCCC	50.0	55.0	58.2	58.3	1575-1811 bp	1.0	82.1
petN-rpoB_p3	AGCCCAAGCGAGACTTACTA	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.2	58.4	1559-1795 bp	1.0	81.4
petN-rpoB_p4	AGCCCAAGCGAGACTTACTA	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.2	57.6	1560-1796 bp	1.0	81.2
petN-rpoB_p5	CCACTCCTCCCCCATACTAC	CGGGTATTGTGGACATTCCC	60.0	55.0	58.3	58.3	1631-1867 bp	1.0	81.0