Hotspot

trnD-GUC-psbM

LSC · 16210-17261 · 1052 bp

Back to Themeda

Pi 0.00717

MarkerSeek score 58.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 27
pi: 0.00717
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	1094-1191 bp	1.0	57.4
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	58.5	57.7	1095-1192 bp	1.0	55.3
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	AGGCAGTAGGAACTAGAATGA	50.0	42.9	58.5	55.5	1095-1192 bp	1.0	54.1
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	50.0	40.0	58.5	54.5	1055-1152 bp	1.0	54.0
trnD-GUC-psbM_p5	GTTCAATTGGTCAGAGCACC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.0	57.9	1095-1192 bp	1.0	53.2