Hotspot

trnG-UCC-trnM-CAU

LSC · 13672-14970 · 1299 bp

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Pi 0.00751

MarkerSeek score 58.20

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1299
variable_sites: 36
pi: 0.00751
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	TCGAACCGATGACTTCTGTC	38.1	50.0	55.5	57.4	1415-2127 bp	1.0	51.3
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	CGAACCGATGACTTCTGTCT	38.1	50.0	55.5	57.4	1414-2126 bp	1.0	51.2
trnG-UCC-trnM-CAU_p3	AGAACGAATCACACTTTTACCAC	TCGAACCGATGACTTCTGTC	39.1	50.0	57.3	57.4	1418-2130 bp	1.0	50.2
trnG-UCC-trnM-CAU_p4	GAACGAATCACACTTTTACCACT	TCGAACCGATGACTTCTGTC	39.1	50.0	57.3	57.4	1417-2129 bp	1.0	50.2
trnG-UCC-trnM-CAU_p5	GAACGAATCACACTTTTACCACT	CGAACCGATGACTTCTGTCT	39.1	50.0	57.3	57.4	1416-2128 bp	1.0	50.1