Hotspot

trnK-UUU-rps16

LSC · 3914-4469 · 556 bp

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Pi 0.0139
MarkerSeek score 53.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
556
variable_sites
22
pi
0.0139
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGAGGATCAGTCGTGGTCTT TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.1 58.2 965-978 bp 1.0 81.2
trnK-UUU-rps16_p2 TGAGGATCAGTCGTGGTCTT TTTCTCGAGCCGTATGAGGA 50.0 50.0 58.1 58.2 969-982 bp 1.0 81.2
trnK-UUU-rps16_p3 AACCTTTCCCTGCATTAGGC TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.1 58.2 1194-1210 bp 1.0 81.2
trnK-UUU-rps16_p4 AACCTTTCCCTGCATTAGGC TTTCTCGAGCCGTATGAGGA 50.0 50.0 58.1 58.2 1198-1214 bp 1.0 81.1
trnK-UUU-rps16_p5 ACCCAGGAAAGAAAGGGAGT TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.2 58.2 1460-1476 bp 1.0 80.5