Hotspot

petB

LSC · 71323-72816 · 1494 bp

Back to Thelypteris

Pi 0.0129

MarkerSeek score 52.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1494
variable_sites: 29
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTTGCCATTTTCTTCCGGGA	AAGAAAGGTCGGACAGGAGA	45.0	50.0	58.0	58.0	2476-2610 bp	1.0	81.4
petB_p2	TTTGCCATTTTCTTCCGGGA	TTGTTTTGCCCCAGTAGTCC	45.0	50.0	58.0	58.0	2349-2483 bp	1.0	81.3
petB_p3	TTTGCCATTTTCTTCCGGGA	CGGGGGAAATGTATGCATCA	45.0	50.0	58.0	58.0	2660-2794 bp	1.0	81.2
petB_p4	TTTGCCATTTTCTTCCGGGA	CTCATACGGCTCAAGCAAGT	45.0	50.0	58.0	58.0	2971-2974 bp	0.666667	67.6
petB_p5	TTTGCCATTTTCTTCCGGGA	TGATGCGGGGGAAATGTATG	45.0	50.0	58.0	58.0	2668-2799 bp	0.666667	67.5