Hotspot

ycf1-chlN

SSC · 124149-125627 · 1479 bp

Back to Thelypteris

Pi 0.0183

MarkerSeek score 57.10

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 1479
variable_sites: 39
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	CTTTCGCCGACAAAGTTTCC	GGGAGATGAAGAAGCCCATG	50.0	55.0	58.0	58.0	2106-2115 bp	1.0	82.1
ycf1-chlN_p2	AAAAATGTTTGTGGCACCGG	GGGAGATGAAGAAGCCCATG	45.0	55.0	58.1	58.0	1918-1927 bp	1.0	82.1
ycf1-chlN_p3	CAACAACTTGACTCGACCCA	GGGAGATGAAGAAGCCCATG	50.0	55.0	58.1	58.0	1781-1790 bp	1.0	82.0
ycf1-chlN_p4	CTTTCGCCGACAAAGTTTCC	ACTCTATTTTAGGCTGCCGC	50.0	50.0	58.0	58.0	1866-1875 bp	1.0	82.0
ycf1-chlN_p5	CTTTCGCCGACAAAGTTTCC	GAACAGCGACAACACCAATG	50.0	50.0	58.0	58.0	2457 bp	0.666667	67.3