Hotspot

trnP-GGG-trnL-UAG

SSC · 110160-110425 · 266 bp

Back to Thelypteris

Pi 0.0451

MarkerSeek score 73.60

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: SSC
length_bp: 266
variable_sites: 18
pi: 0.0451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GATTCGAACCTGTGACCTCC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	381-401 bp	1.0	84.2
trnP-GGG-trnL-UAG_p2	GATTCGAACCTGTGACCTCC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	379-399 bp	1.0	84.2
trnP-GGG-trnL-UAG_p3	GGATTCGAACCTGTGACCTC	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.0	58.0	382-402 bp	1.0	84.2
trnP-GGG-trnL-UAG_p4	GGATTCGAACCTGTGACCTC	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.0	58.0	380-400 bp	1.0	84.2
trnP-GGG-trnL-UAG_p5	GATTCGAACCTGTGACCTCC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	368-388 bp	1.0	83.5