Hotspot

trnN-GUU-rpl21

SSC · 105693-108711 · 3019 bp

Back to Thelypteris

Pi 0.0148
MarkerSeek score 56.50
Primer available yes
Label trnN-GUU-rpl21

Feature Metadata

label_name
trnN-GUU-rpl21
feature_type
igs
region
SSC
length_bp
3019
variable_sites
67
pi
0.0148
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl21_p1 AGTGAAGGGATTGAACTAGT TTGCCTCTGATTGTTTTGGC 40.0 45.0 53.2 57.2 188 bp 1.0 44.3
trnN-GUU-rpl21_p2 AGTGAAGGGATTGAACTAGT TTGCATGGTATTGCCTCTGA 40.0 45.0 53.2 57.2 198 bp 1.0 44.0
trnN-GUU-rpl21_p3 AAGTGAAGGGATTGAACTAGT TTGCATGGTATTGCCTCTGA 38.1 45.0 54.2 57.2 199 bp 1.0 44.0
trnN-GUU-rpl21_p4 ACTAAGTGAAGGGATTGAACT TTGCATGGTATTGCCTCTGA 38.1 45.0 54.2 57.2 202 bp 1.0 44.0
trnN-GUU-rpl21_p5 AGTGAAGGGATTGAACTAGTT TTGCATGGTATTGCCTCTGA 38.1 45.0 54.2 57.2 198 bp 1.0 44.0