Hotspot

trnP-UGG-psaJ

LSC · 63201-63554 · 354 bp

Back to Thelypteris

Pi 0.0235

MarkerSeek score 63.30

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 12
pi: 0.0235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CCCAAAACAAACACGCTACC	ACAGGCCAAACCATATTGCA	50.0	45.0	57.9	58.1	456-460 bp	1.0	80.9
trnP-UGG-psaJ_p2	CCCAAAACAAACACGCTACC	TTAACGAACCGGCTAACAGG	50.0	50.0	57.9	57.9	471-475 bp	1.0	80.7
trnP-UGG-psaJ_p3	CCCAAAACAAACACGCTACC	AGGAGCCGTGGAAAGGTATA	50.0	50.0	57.9	57.8	428-432 bp	1.0	80.2
trnP-UGG-psaJ_p4	CCAAAACAAACACGCTACCG	ACAGGCCAAACCATATTGCA	50.0	45.0	58.3	58.1	455-459 bp	1.0	79.9
trnP-UGG-psaJ_p5	CCAAAACAAACACGCTACCG	TTAACGAACCGGCTAACAGG	50.0	50.0	58.3	57.9	470-474 bp	1.0	79.7