Hotspot

ycf4-cemA

LSC · 57000-57182 · 183 bp

Back to Thelypteris

Pi 0.0437

MarkerSeek score 70.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 11
pi: 0.0437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TCCGCGTGTATCGATTGAAG	GCTTCCAGTAATGTAGTCGCA	50.0	47.6	58.2	58.1	228-233 bp	1.0	75.3
ycf4-cemA_p2	TCCGCGTGTATCGATTGAAG	TGCTTCCAGTAATGTAGTCGC	50.0	47.6	58.2	58.1	229-234 bp	1.0	75.3
ycf4-cemA_p3	TTCCGCGTGTATCGATTGAA	GCTTCCAGTAATGTAGTCGCA	45.0	47.6	57.7	58.1	229-234 bp	1.0	74.4
ycf4-cemA_p4	TTCCGCGTGTATCGATTGAA	TGCTTCCAGTAATGTAGTCGC	45.0	47.6	57.7	58.1	230-235 bp	1.0	74.3
ycf4-cemA_p5	TTTCCGCGTGTATCGATTGA	GCTTCCAGTAATGTAGTCGCA	45.0	47.6	57.7	58.1	230-235 bp	1.0	74.3