Hotspot

ndhJ-ndhK

LSC · 47241-47310 · 70 bp

Back to Thelypteris

Pi 0.0381

MarkerSeek score 70.50

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 4
pi: 0.0381
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	AGTTGGCCAAGCGGATAATC	CGGAAAACTTTCAAAAGCTTGG	50.0	40.9	58.3	57.2	177 bp	1.0	57.0
ndhJ-ndhK_p2	CTAGTTGGCCAAGCGGATAA	CGGAAAACTTTCAAAAGCTTGG	50.0	40.9	57.7	57.2	179 bp	1.0	57.0
ndhJ-ndhK_p3	CCGCGTCTCGACATTAAACT	CGGAAAACTTTCAAAAGCTTGG	50.0	40.9	58.4	57.2	153 bp	1.0	56.9
ndhJ-ndhK_p4	CCAAAGGTCGGTGGGAAAAA	CGGAAAACTTTCAAAAGCTTGG	50.0	40.9	58.6	57.2	205 bp	1.0	54.6
ndhJ-ndhK_p5	AGTTGGCCAAGCGGATAATC	TCTCGGAAAACTTTCAAAAGCT	50.0	36.4	58.3	56.8	180 bp	1.0	53.2