Hotspot

trnS-UGA-psbC

LSC · 31336-31633 · 298 bp

Back to Thelypteris

Pi 0.0382

MarkerSeek score 71.70

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 15
pi: 0.0382
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GTCTTGAAAACTGGCATGGC	TTCTTCTTTTTCGTGGGCCA	50.0	45.0	57.9	57.9	477-486 bp	1.0	83.1
trnS-UGA-psbC_p2	GGGTTCGAATCCCTCTCTCT	TTCTTCTTTTTCGTGGGCCA	55.0	45.0	58.2	57.9	441-450 bp	1.0	82.3
trnS-UGA-psbC_p3	GTCTTGAAAACTGGCATGGC	TCTTCTTTTTCGTGGGCCAT	50.0	45.0	57.9	57.7	476-485 bp	1.0	81.3
trnS-UGA-psbC_p4	GAGATGGTCGAGCGGTTTAA	TTCTTCTTTTTCGTGGGCCA	50.0	45.0	57.7	57.9	504-513 bp	1.0	81.1
trnS-UGA-psbC_p5	AGATGGTCGAGCGGTTTAAG	TTCTTCTTTTTCGTGGGCCA	50.0	45.0	57.7	57.9	503-512 bp	1.0	81.1