Hotspot

psbZ-trnS-UGA

LSC · 30814-31246 · 433 bp

Back to Thelypteris

Pi 0.02

MarkerSeek score 62.40

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 13
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	AGCTAATTGGAACGCGATAGT	GCCATGCCAGTTTTCAAGAC	42.9	50.0	57.5	57.9	487-508 bp	1.0	73.2
psbZ-trnS-UGA_p2	AGCTAATTGGAACGCGATAGT	TTAAACCGCTCGACCATCTC	42.9	50.0	57.5	57.7	460-481 bp	1.0	71.8
psbZ-trnS-UGA_p3	AGCTAATTGGAACGCGATAGT	CTTAAACCGCTCGACCATCT	42.9	50.0	57.5	57.7	461-482 bp	1.0	71.7
psbZ-trnS-UGA_p4	AGCTAATTGGAACGCGATAGT	TGCCATGCCAGTTTTCAAGA	42.9	45.0	57.5	58.3	488-509 bp	1.0	71.4
psbZ-trnS-UGA_p5	GCTAATTGGAACGCGATAGTC	GCCATGCCAGTTTTCAAGAC	47.6	50.0	57.2	57.9	486-507 bp	1.0	70.7