Hotspot

trnG-GCC-psbZ

LSC · 30358-30624 · 267 bp

Back to Thelypteris

Pi 0.0331

MarkerSeek score 73.90

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 13
pi: 0.0331
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	TCTGTGGGATAGGCGGATAG	TTGTCTTTTCTGGGGCTTCA	55.0	45.0	58.1	57.3	410-423 bp	1.0	78.4
trnG-GCC-psbZ_p2	ACCTCTTCGTTTTCTGTGGG	TTGTCTTTTCTGGGGCTTCA	50.0	45.0	57.7	57.3	422-435 bp	1.0	76.8
trnG-GCC-psbZ_p3	TCTGTGGGATAGGCGGATAG	TGTCTTTTCTGGGGCTTCAT	55.0	45.0	58.1	57.0	409-422 bp	1.0	76.6
trnG-GCC-psbZ_p4	TCTGTGGGATAGGCGGATAG	TGGGGCTTCATTATGGATTGG	55.0	47.6	58.1	58.0	400-413 bp	1.0	76.2
trnG-GCC-psbZ_p5	TTCTGTGGGATAGGCGGATA	TTGTCTTTTCTGGGGCTTCA	50.0	45.0	57.6	57.3	411-424 bp	1.0	75.9