Hotspot

rpoC2-trnD-GUC

LSC · 20041-27355 · 7315 bp

Back to Thelypteris

Pi 0.00789
MarkerSeek score 45.80
Primer available yes
Label rpoC2-trnD-GUC

Feature Metadata

label_name
rpoC2-trnD-GUC
feature_type
igs
region
LSC
length_bp
7315
variable_sites
85
pi
0.00789
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-trnD-GUC_p1 CAATCCACCCCTAGCCTCTA TTTCCTTTACAGCCTGCGAG 55.0 50.0 57.9 58.2 162 bp 1.0 83.1
rpoC2-trnD-GUC_p2 GAAACAATCCACCCCTAGCC TTTCCTTTACAGCCTGCGAG 55.0 50.0 58.2 58.2 166 bp 1.0 81.9
rpoC2-trnD-GUC_p3 CAATCCACCCCTAGCCTCTA ATTTCCTTTACAGCCTGCGA 55.0 45.0 57.9 57.5 163 bp 1.0 80.7
rpoC2-trnD-GUC_p4 TGAAACAATCCACCCCTAGC TTTCCTTTACAGCCTGCGAG 50.0 50.0 57.5 58.2 167 bp 1.0 79.7
rpoC2-trnD-GUC_p5 GAAACAATCCACCCCTAGCC ATTTCCTTTACAGCCTGCGA 55.0 45.0 58.2 57.5 167 bp 1.0 79.5