Hotspot

psbK-psbI

LSC · 7222-7755 · 534 bp

Back to Thelypteris

Pi 0.0262

MarkerSeek score 63.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 21
pi: 0.0262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCAGTTAGTTTTCG	AGGGTTTCGTCCAGGATCAT	50.0	50.0	58.0	58.4	654-666 bp	1.0	78.8
psbK-psbI_p2	CAAGCCGCAGTTAGTTTTCG	GGGTTTCGTCCAGGATCATT	50.0	50.0	58.0	57.6	653-665 bp	1.0	78.8
psbK-psbI_p3	CAAGCCGCAGTTAGTTTTCG	TTTTACCTAATCCCCACGGC	50.0	50.0	58.0	57.6	675-687 bp	1.0	78.7
psbK-psbI_p4	CGGTGTTCTTTCTCCTCCTG	AGGGTTTCGTCCAGGATCAT	55.0	50.0	57.9	58.4	686-698 bp	1.0	78.4
psbK-psbI_p5	CGGTGTTCTTTCTCCTCCTG	GGGTTTCGTCCAGGATCATT	55.0	50.0	57.9	57.6	685-697 bp	1.0	78.4