Hotspot

matK-rps16

LSC · 2686-3643 · 958 bp

Back to Thelypteris

Pi 0.0202

MarkerSeek score 57.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 29
pi: 0.0202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	AGTGGATGCACAAACTTCGT	GTTTTACCCCGTCTACGACC	45.0	55.0	58.0	58.0	2211-2216 bp	1.0	81.9
matK-rps16_p2	AGTGGATGCACAAACTTCGT	TAATTGCGATCGACAGCCAA	45.0	45.0	58.0	58.0	1709-1711 bp	1.0	81.7
matK-rps16_p3	AGTGGATGCACAAACTTCGT	AAGCTATCAGGGAGGTTGGT	45.0	50.0	58.0	58.0	1673-1675 bp	1.0	81.6
matK-rps16_p4	AGTGGATGCACAAACTTCGT	AGCTATCAGGGAGGTTGGTT	45.0	50.0	58.0	58.0	1672-1674 bp	1.0	81.6
matK-rps16_p5	AGTGGATGCACAAACTTCGT	GTGGTATTTTGAAACGGGCG	45.0	50.0	58.0	58.0	1562-1564 bp	1.0	81.6