Hotspot

atpF

LSC · 11696-13049 · 1354 bp

Back to Thamnosma

Pi 0.0163

MarkerSeek score 68.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1354
variable_sites: 22
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATTGTTGCC	AGTCGATCTAAAAGAGGAGATCA	50.0	39.1	58.4	56.3	1459-1468 bp	1.0	41.5
atpF_p2	GCTCAATACGCTCACGGATA	AGTCGATCTAAAAGAGGAGATCA	50.0	39.1	57.6	56.3	1492-1501 bp	1.0	41.5
atpF_p3	TCGTCGGCTTTAATTGTTGC	AGTCGATCTAAAAGAGGAGATCA	45.0	39.1	57.4	56.3	1460-1469 bp	1.0	41.5
atpF_p4	ATTGCTCAATACGCTCACGG	AGTCGATCTAAAAGAGGAGATCA	50.0	39.1	58.7	56.3	1495-1504 bp	1.0	41.5
atpF_p5	GTCGGCTTTAATTGTTGCCA	AGTCGATCTAAAAGAGGAGATCA	45.0	39.1	57.3	56.3	1458-1467 bp	1.0	41.5