Hotspot

rpl32-trnL-UAG

SSC · 113987-114671 · 685 bp

Back to Thamnosma

Pi 0.0365

MarkerSeek score 70.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 685
variable_sites: 20
pi: 0.0365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGAAATCCCTTTCTACGGGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	645-779 bp	1.0	85.8
rpl32-trnL-UAG_p2	CGAAATCCCTTTCTACGGGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	646-780 bp	1.0	85.8
rpl32-trnL-UAG_p3	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	667-801 bp	1.0	84.8
rpl32-trnL-UAG_p4	GCGTTGAAGGCTTTTTCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	668-802 bp	1.0	84.8
rpl32-trnL-UAG_p5	CGAAATCCCTTTCTACGGGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	655-789 bp	1.0	84.8