Hotspot

rps3-rpl22

LSC · 83552-83619 · 68 bp

Back to Thamnosma

Pi 0.0588

MarkerSeek score 77.20

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 4
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAAGTCGGAAACCAAGTGGA	ATCAAAAGACCCACTTGCCA	50.0	45.0	58.0	57.6	192-203 bp	1.0	78.3
rps3-rpl22_p2	CAAGTCGGAAACCAAGTGGA	TCAAAAGACCCACTTGCCAT	50.0	45.0	58.0	57.6	191-202 bp	1.0	78.3
rps3-rpl22_p3	GATGGCTTTGGGTTGTACCA	ATCAAAAGACCCACTTGCCA	50.0	45.0	58.1	57.6	210-221 bp	1.0	77.8
rps3-rpl22_p4	GATGGCTTTGGGTTGTACCA	TCAAAAGACCCACTTGCCAT	50.0	45.0	58.1	57.6	209-220 bp	1.0	77.8
rps3-rpl22_p5	TGATGGCTTTGGGTTGTACC	TCAAAAGACCCACTTGCCAT	50.0	45.0	58.1	57.6	210-221 bp	1.0	77.8