Hotspot

petA-psbJ

LSC · 63428-64244 · 817 bp

Back to Thamnosma

Pi 0.016
MarkerSeek score 68.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
817
variable_sites
13
pi
0.016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC AGCTGGTATTGCTGTGATCG 50.0 50.0 58.0 58.1 1012-1020 bp 1.0 80.5
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.0 58.1 959-967 bp 1.0 80.5
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC TCCGGATTAGGTTCGTCCTT 50.0 50.0 58.0 58.1 960-968 bp 1.0 79.9
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT AGCTGGTATTGCTGTGATCG 50.0 50.0 58.2 58.1 1009-1017 bp 1.0 79.4
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT CCGGATTAGGTTCGTCCTTG 50.0 55.0 58.2 58.1 956-964 bp 1.0 79.4