Hotspot

accD-psaI

LSC · 59164-59907 · 744 bp

Back to Thamnosma

Pi 0.0244

MarkerSeek score 58.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 18
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGTGAACAATGTGGCCATCA	ATTTGACCTTGGACCGATCG	45.0	50.0	58.0	58.0	1611-1617 bp	1.0	81.2
accD-psaI_p2	CAAACAGGTACAGGGCAACT	ATTTGACCTTGGACCGATCG	50.0	50.0	58.0	58.0	1419-1425 bp	1.0	81.2
accD-psaI_p3	GTTCAAACAGGTACAGGGCA	ATTTGACCTTGGACCGATCG	50.0	50.0	58.0	58.0	1422-1428 bp	1.0	81.2
accD-psaI_p4	TCAAACAGGTACAGGGCAAC	ATTTGACCTTGGACCGATCG	50.0	50.0	58.0	58.0	1420-1426 bp	1.0	81.2
accD-psaI_p5	TGTTCAAACAGGTACAGGGC	ATTTGACCTTGGACCGATCG	50.0	50.0	58.0	58.0	1423-1429 bp	1.0	81.2