Hotspot

ycf3-trnS-GGA

LSC · 45605-45960 · 356 bp

Back to Thamnosma

Pi 0.0291

MarkerSeek score 66.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 10
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTGATCCGCGATCTAGG	ACCCCCGGTAAACAAAAGTC	55.0	50.0	58.0	58.0	447-495 bp	1.0	83.6
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	ACCCCCGGTAAACAAAAGTC	50.0	50.0	58.1	58.0	519-567 bp	1.0	82.6
ycf3-trnS-GGA_p3	CCATTGATCCGCGATCTAGG	ACGGAAAGAGAGGGATTCGA	55.0	50.0	58.0	58.2	467-515 bp	1.0	82.4
ycf3-trnS-GGA_p4	CCATTGATCCGCGATCTAGG	TTCCAATGCTACGCCTTGAA	55.0	45.0	58.0	57.8	417-465 bp	1.0	82.4
ycf3-trnS-GGA_p5	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	539-587 bp	1.0	81.4