Hotspot

psbZ-trnG-UCC

LSC · 36879-37440 · 562 bp

Back to Thamnosma

Pi 0.016

MarkerSeek score 65.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 9
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GTATTTGCCTCTCCGGATGG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.1	58.1	734-757 bp	1.0	79.6
psbZ-trnG-UCC_p2	GTATTTGCCTCTCCGGATGG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.1	58.1	735-758 bp	1.0	79.6
psbZ-trnG-UCC_p3	TCCGGATGGTTGGTCAAGTA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.4	58.1	723-746 bp	1.0	77.7
psbZ-trnG-UCC_p4	TCCGGATGGTTGGTCAAGTA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.4	58.1	724-747 bp	1.0	77.7
psbZ-trnG-UCC_p5	GTTGTATTTGCCTCTCCGGA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.6	58.1	737-760 bp	1.0	77.3