Hotspot

trnT-GGU-psbD

LSC · 32378-33573 · 1196 bp

Back to Thamnosma

Pi 0.0186
MarkerSeek score 67.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1196
variable_sites
22
pi
0.0186
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAGCAATAGACCGGACCAAC 55.0 50.0 58.1 57.9 1363-1368 bp 1.0 80.7
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA AAGCAATAGACCGGACCAAC 50.0 50.0 58.1 57.9 1366-1371 bp 1.0 80.5
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG AAAAACGAATCGGTCCCTCC 55.0 50.0 58.1 58.2 1339-1344 bp 1.0 80.1
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA AAAAACGAATCGGTCCCTCC 50.0 50.0 58.1 58.2 1342-1347 bp 1.0 79.9
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1355-1360 bp 1.0 79.5