Hotspot

trnS-GCU-trnG-GCC

LSC · 8167-8880 · 714 bp

Back to Thamnosma

Pi 0.0244

MarkerSeek score 66.80

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 17
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CCGACGCTTTAATCCACTCA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	779-782 bp	1.0	56.0
trnS-GCU-trnG-GCC_p2	CCGACGCTTTAATCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	776-779 bp	1.0	55.1
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.6	837-840 bp	1.0	54.6
trnS-GCU-trnG-GCC_p4	CCGACGCTTTAATCCACTCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	778-781 bp	1.0	54.0
trnS-GCU-trnG-GCC_p5	ACGCTTTAATCCACTCAGCC	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.3	57.6	776-779 bp	1.0	54.0