Hotspot

trnK-UUU-rps16

LSC · 4276-4571 · 296 bp

Back to Thamnosma

Pi 0.0237

MarkerSeek score 51.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 7
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	TGTGCCGTTTATCGAATCGT	55.0	45.0	58.0	58.0	653-657 bp	1.0	82.4
trnK-UUU-rps16_p2	TTCCACGCCAAGACTTCAAA	TGTGCCGTTTATCGAATCGT	45.0	45.0	58.0	58.0	754-758 bp	1.0	81.9
trnK-UUU-rps16_p3	ACTCGACCCAACCTGGATTA	TGTGCCGTTTATCGAATCGT	50.0	45.0	58.1	58.0	785-789 bp	1.0	81.8
trnK-UUU-rps16_p4	GGTAAGGGATTTAGTGGGCG	TGTGCCGTTTATCGAATCGT	55.0	45.0	58.0	58.0	1251-1255 bp	1.0	81.2
trnK-UUU-rps16_p5	AGTGGGCGATCAAACGATAC	TGTGCCGTTTATCGAATCGT	50.0	45.0	58.1	58.0	1239-1243 bp	1.0	81.0