Hotspot

rps18

LSC · 68319-68810 · 492 bp

Back to Thamnocalamus

Pi 0

MarkerSeek score 60.60

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 492
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGACTACTTCTTCTGGATCCA	GGTCTGGCCCTTTCGAATAA	42.9	50.0	55.1	57.6	573-594 bp	1.0	48.6
rps18_p2	AGACTACTTCTTCTGGATCCA	AGGTCTGGCCCTTTCGAATA	42.9	50.0	55.1	58.4	574-595 bp	1.0	48.6
rps18_p3	AGACTACTTCTTCTGGATCCA	ATAGGTCTGGCCCTTTCGAA	42.9	50.0	55.1	58.4	576-597 bp	1.0	48.6
rps18_p4	AGACTACTTCTTCTGGATCCA	TAGGTCTGGCCCTTTCGAAT	42.9	50.0	55.1	58.4	575-596 bp	1.0	48.6
rps18_p5	TGGACCAAAGAAAGACTACTTCT	GGTCTGGCCCTTTCGAATAA	39.1	50.0	57.1	57.6	585-606 bp	1.0	48.4