Hotspot

ndhC-trnV-UAC

LSC · 52565-53333 · 769 bp

Back to Thamnocalamus

Pi 0.0052
MarkerSeek score 61.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
769
variable_sites
4
pi
0.0052
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 902-972 bp 1.0 76.0
ndhC-trnV-UAC_p2 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.4 57.9 906-976 bp 1.0 74.9
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.5 58.3 903-973 bp 1.0 74.2
ndhC-trnV-UAC_p4 TGGTCCTTCACTAATCGGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.8 57.9 905-975 bp 1.0 73.3
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG CGGTTCGAATCCGTATAGCC 55.0 55.0 57.4 58.3 907-977 bp 1.0 73.1