Hotspot

ycf3-trnS-GGA

LSC · 46475-47064 · 590 bp

Back to Thamnocalamus

Pi 0.00678

MarkerSeek score 62.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 4
pi: 0.00678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	777-778 bp	1.0	79.0
ycf3-trnS-GGA_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	727-728 bp	1.0	78.7
ycf3-trnS-GGA_p3	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.0	58.5	729-730 bp	1.0	75.9
ycf3-trnS-GGA_p4	TTTTTCCCCTGAGGTTGTCG	CAGTTCCAATGCTACGCCTT	50.0	50.0	58.0	58.5	730-731 bp	1.0	75.9
ycf3-trnS-GGA_p5	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	50.0	50.0	58.0	58.6	726-727 bp	1.0	75.8