Hotspot

trnE-UUC-trnY-GUA

LSC · 17047-17107 · 61 bp

Back to Thamnocalamus

Pi 0.0164

MarkerSeek score 60.70

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 1
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	CGTCTAGTGAAGTGATGCCC	CTAGATTTTTGGGCCGAGCT	55.0	50.0	58.1	58.0	243 bp	1.0	79.4
trnE-UUC-trnY-GUA_p2	GTGAAGTGATGCCCCTATCG	CTAGATTTTTGGGCCGAGCT	55.0	50.0	58.1	58.0	237 bp	1.0	79.0
trnE-UUC-trnY-GUA_p3	GAAGTGATGCCCCTATCGTC	CTAGATTTTTGGGCCGAGCT	55.0	50.0	57.8	58.0	235 bp	1.0	78.7
trnE-UUC-trnY-GUA_p4	AAGTGATGCCCCTATCGTCT	CTAGATTTTTGGGCCGAGCT	50.0	50.0	58.2	58.0	234 bp	1.0	78.3
trnE-UUC-trnY-GUA_p5	TCTAGTGAAGTGATGCCCCT	CTAGATTTTTGGGCCGAGCT	50.0	50.0	57.7	58.0	241 bp	1.0	77.9